In the shocking clip, which was posted to YouTube on Monday, high-spirited demonstrators apparently use the Libyan dictator's corpse as a ventriloquist's doll.
The footage came to light after a Syrian activist linked to it on Twitter, aiming a threat at Syria's leader Bashar Al Assad.
The minute-long video, whose authenticity cannot be independently verified, seems to have been filmed on October 20 last year, the day Gaddafi was captured and killed by rebel troops.
It shows a confused scene of celebration as young men shout and cheer next to a van containing an apparently dead body which resembles the dictator.
The corpse is topless and covered in blood, and is apparently limp.
One rebel grabs its head and manipulates it while talking in Arabic, apparently mocking the dead leader.
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