Karuna Sanusan, 24, carried out the bloody attack on 40-year-old Siripan after discovering he was having an affair, she told police.
She said she then hurled his severed manhood out the bedroom window, leaving Siripan writhing and screaming in agony in a pool of his own blood.
Neighbors heard his howls of pain and called the police, who arrived at the couple's home in Sriracha, near Bangkok, Thailand, at 6:20 a.m.
Officers recovered his penis and put it in a cooler for surgeons to try to reattach — but medics said that would not be possible.
Siripan was rushed to the nearby Samitivej Sriracha Hospital while Karuna was arrested and held in custody.