The head of state claims the coalition has given support to terror groups including ISIS and Kurdish militant groups YPG and PYD.
Erdogan said "it's very clear" the US is supporting terror groups in a damning press conference in Ankara.
He said: "They were accusing us of supporting Daesh (Islamic State).
"Now they give support to terrorist groups including Daesh, YPG, PYD. It's very clear. We have confirmed evidence, with pictures, photos and videos."
American-led forces have worked alongside Syrian rebels fighting Bashar al-Assad, but have avoided helping ISIS and other militant groups.