If the measure passes, Ohio would become the fifth state and the first in the Midwest to legalize the recreational use of marijuana, in addition to the District of Columbia.
A pro-marijuana group, ResponsibleOhio, gathered enough signatures to place the measure on the upcoming ballot, Secretary of State Jon Husted said in a statement.
The group needed 305,267 signatures and got 320,267 after initially coming up short in June.
The amendment would legalize medical marijuana and the use of up to one ounce for adults 21 years or older. Limited amounts of home growing would also be allowed.
"It's time for marijuana legalization in Ohio, and voters will have the opportunity to make it happen this November," ResponsibleOhio Executive Director Ian James said in a statement.